Characterisation of arabinogalactan endo β 1,4 galactanases from Globodera rostochiensis, Globodera pallida and Rotylenchulus reniformis

Plant parasitic nematodes need to overcome the barrier presented by plant cell wall in order invade their host. A variety of degrading enzymes are present endoparasitic including that degrade cellulose (beta 1,4 endoglucanases) and various pectin components. We describe cloning functional analysis genes encoding GH53 arabinogalactan endo-1,4-beta-galactosidases from three related Globodera rostochiensis, pallida Rotylenchulus reniformis . Phylogenetic structural analyses strongly indicate these have been acquired horizontal gene transfer bacteria. show expressed at invasive stages parasites secretory gland cells. also demonstrate species biochemically active, showing expected hydrolytic enzymatic activity when galactan was used as a substrate. This work further demonstrates importance degradation success process extensive role has played evolution parasitism nematodes. • Some plant-parasitic nematodes, cyst species, produce endo β galactanases. The proteins pharyngeal cells where they can be secreted into host plant. These early nematode life cycle, consistent with host-parasite interaction. encoded specifically metabolise